Chromatographic systems size exclusion

MAJOR APPLICATIONS Membranes, chromatographic resins, size exclusion particles, drug delivery systems. 

Fig. 1. Classification of chromatographic systems where gsc is gas—soHd chromatography glc, gas—Hquid chromatography sec, size-exclusion chromatography Isc, Hquid—soHd chromatography Uc, Hquid—Hquid chromatography iec, ion-exchange chromatography tic, thin-layer chromatography ...

This presentation demonstrates that a small minicomputer can be used to provide a full range of functions for collection and interactive reduction of data from a size-exclusion liquid chromatograph. A number of different users have collected in excess of 5000 chromatograms using this equipment. The experience gained with this system has influenced our approach to the automation of other analytical instruments. Careful attention to control paths, provision of "user friendly" access to the system functions, and careful management of the data archiving functions are crucial to the success of such efforts. 

A more sensitive viscometer than the drop-time glass capillary method is also needed in size exclusion chromatography (SEC) such as the gel permeation chromatographic (GPC) analysis of polymer molecular weight distribution (MWD). In an SEC system, a concentration detector is commonly used for providing the weight concentration profile of the polymer elution curve. 

The automation of the SEC/Viscometer detector system is achieved by interfacing the DRI and viscosity detectors to a microcomputer for real time data acquisition. The raw data subsequently are transferred to a minicomputer (DEC PDP-11/44) for storage and data reduction. The automated data acquisition and analysis system for the size exclusion chromatograph with multiple detectors used in this study have been described previously.(23)... 

A way out of the dilemma is the determination of the molecular size distribution of the chromatographable organic carbon (COC) as it is offered from size exclusion chromatography (SEC) equipped with an organic carbon (OC) detection system. 

---