Chromatographic suitability capacity factor

Standards and blanks are the usual controls used in analytical HPLC. Standards are usually interspersed with samples to demonstrate system performance over the course of a batch run. The successful run of standards before beginning analysis demonstrates that the system is suitable to use. In this way, no samples are run until the system is working well. Typically, standards are used to calculate column plate heights, capacity factors, and relative response factors. If day-to-day variability has been established by validation, the chromatographic system can be demonstrated to be within established control limits. One characteristic of good science is that samples... 

Chromatographic system (see Chromatography, in the general procedure (621)) The liquid chromatography is equipped with a 280-nm detector and a 4.6 mm x 15-cm column that contains 5-/im packing L7. The flow-rate is about 0.8 ml/min. Chromatograph the System suitability solution, and record the peak responses as directed for Procedure the capacity factor, k , is not less than 6 the column efficiency is not less than 3000 theoretical plates the tailing factor is not more than 1.5 and the relative standard deviation (RSD) for replicate injections is not more than 1%. 

By input compounds names or chemical formulas to RPS, suitable descriptors for the compounds group desired are calculated with the same procedures as in the main function of RPS by the computer and then capacity factor s for the solutes at v u ious mobile phase compositions are predicted by step-by-step with the interval of X=0.01 for both aqueous acetonitrile and methanol mobile phases. The range available in this procedure is from 0.3 to 0.7 of X-values for acetonitrile system and from 0.4 to 0.8 for methanol system, respectively. After calculations of capacity factors for the desired solutes, Rgand Tp, for each step are estimated according to the equation-9 and 10, at five different flow rates of the mobile phase such as 1, 2, 4, 8 and 16 uL/min (because we use microcolumns) and then quality of the separation is Judged using a simple numerical chromatographic response function (CRF) defined as follows ... 

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