Chromatographic Properties of Polymer Monolithic Columns

the methacrylate-based monolithic columns showed retention behavior similar to that of the silica columns by HPLC in 70% ACN, although methylene selectivity (01CH2) was lower. The results were attributed to the lower surface hydrophobicity of the polymeric columns having polar ester groups. The van Deem ter curves showed that the efficiency of the columns for homologous alkylbenzenes was lower than that of silica columns. 

The separation efficiency of 25,000/m (plate height 40 pm) was reported for neutral PAH compounds like fluorene. 

Column materials (reference) Skeleton size, (im Macro, (im Meso, nm Surface Area, m2/g HPLC CEC Feed Porosity Observed Porosity Permeabili ty (K), [m2] x 1014 Analytes 

Silica particle0 Particulate, 5 pm Particulate 3 pm NA 9 340 7-19 5-20 NA 0.65 4 Alkylbenzenes, PAHs 

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