Chromatographic processes affinity chromatography

An example of virus clearance factors in chromatographic processes frequently used for purification of antibodies is given in Table 17. Lower clearance factors for protein A affinity chromatography have been found by Mariani and Tarditi128 when compared to results found with protein G by Walter and Allgaier.237 An explanation of this fact can be found in the fact that protein G requires harsher elution conditions than protein A. 

McCoy, B. J. 1991. Theory of affinity chromatography. In Chromatographic and Membrane Processes in Biotechnology (C. A. Costa and J. S. Cabral, eds.), pp. 115-135. Kluwer Academic Publishers, Dordrecht, The Netherlands. 

Arve and Liapis [34] suggest estimating the parameters characterizing the intraparticle diffusion and the adsorption-desorption step mechanisms of affinity chromatography from the experimental data obtained in a batch system. The numerical simulations of the chromatographic process will use the values of the parameters of the adsorption isotherm and those of the effective pore diffusion as determined from stirred tank experiments together with the film mass transfer coefficients calculated from chemical engineering expressions found in the literature. 

In practice, affinity chromatography is usually designed to be the final (if not the sole) chromatographic step in a purification procedure, so as to protect the valuable affinity material from unnecessary contamination. It is normal to use short columns whose capacity is largely fully exploited. Elution can be by a batch process or gradi-... 

Affinity chromatography differs from other chromatographic modes in that a suitable stationary phase can specihcally catch either a single or several components out of a random mix of products owing to a naturally occurring biospecitic bond. A suitable elution process then provides the pure compounds (s). 

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