Chromatographic Parameters and Batch Column Reproducibility

Column evaluations obtained in mobile phases 1 and 3 (see the loading and score plots obtained with mobile phase 1 in Figs. 11 and 12, respectively) with only retention and asymmetry of test compoimds were identical to that previously obtained with the whole set of data. 

Concerning the batch-to-batch and column-to-column reproducibility, the results score plots obtained under each set of chromatographic conditions) showed that new generation supports ensure better batch reproducibility compared to conventional ones. This suggests that only inter-batch reproducibility should be assessed, especially when testing new generation base-deactivated supports. 

This optimization entails a large reduction in the number of measured chromatographic parameters per stationary phase 252 (7 compounds x 3 injections X 3 columns x 2 mobile phases x 2 chromatographic parameters) instead of 1890 (14 compounds x 3 injections x 5 columns x 3 mobile phases x 3 chromatographic parameters). Furthermore, in the analysis of basic compounds, only the mobile phase at pH 7.0 is effectively necessary to achieve a good evaluation of silanol masking capacity of chromatographic supports. A second mobile phase (at pH 3.0) may be required only when an assessment of the hydrophobic properties of stationary phases is needed. 

Score plot obtained in mobile phase 1 with only k and As of seven test compounds. 

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