Chlorpyrifos-oxon systems

Bomser, J.A., Casida, J.E., 2001. Diethylphosphorylation of rat cardiac M2 muscarinic receptor by chlorpyrifos oxon in vitro. Toxicol. Lett. 119 (1), 21-26. Bronstein, A.C., Spyker, D.A., Cantilena Jr., L.R., et al., 2007. 2006 Annual report of the American Association of Poison Control Centers National Poison Data System (NPDS). Clin. Toxicol. (Phila) 45 (8), 815-917. 

In a computer-controlled system the incubation time used was from 1 to 16 min and several minute regeneration with 2-PAM. A system with electric eel AChE was employed for the determination of chlorpyrifos and its metabolites obtaining a detection limit of 24 ppb. The use in the same system of immobilized AChE allows one to improve the detection limit for chlorpyrifos oxon down to 1.1 ppb. In the same system paraoxon and malaoxon were quantified and also determinations of three analytes in spiked river water were carried out. The whole measuring cycle for a single sample with three injections of the substrate prior to inhibition and two after it, incubation and reactivation, took about 40 min. 

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