Chlorophyll a/b complexes

Gradinaru CC, Pascal AA, van Mourik F, Robert B, Horton P, van Grondelle H and van Amerongen H (1998) Ultrafast evolution ofthe excited states in the chlorophyll a/b complex CP29 from green plants studied by energy-selective pump-probe spectroscopy. Biochemistry 37 1143-1149... 

Yang C, Horn R, Paulsen H (2003) The light-harvesting chlorophyll a/b complex can be reconstituted in vitro from its completely unfolded apoprotein. Bioehemistry 42 4527-4533... 

Dockter C, Muller AH, Dietz C, Volkov A, Polyhach Y, Jesehke G, Paulsen H (2012) Rigid core and flexible terminus structure of solubilized light-harvesting chlorophyll a/b complex (LHCII) measured by EPR. J Biol Chem 287 2915-2925. doi 10.1074/jbc.M111.307728... 

Booth PJ, and Paulsen H. Assembly of light harvesting chlorophyll a/b complex in vitro. Time-resolved fluorescence measurements. Biochem 1996 35 5103-5108. 

Hobe S, Prytulla S, Kulhlbrandt W, and Paulsen H. Trimerization and crystallization of reconstimted light-harvesting chlorophyll a/b complex. EMBO 1994 13 3423-3429. 

Garab, G., Faludidaniel, A., Sutherland, J. C., and Hind, G. 1988. Macroorganization of chlorophyll a/b light-harvesting complex in thylakoids and aggregates Information from circular differential scattering. Biochemistry 27 2425-30. 

Simidjiev, L, Barzda, V., Mustardy, L., and Garab, G. 1997. Isolation of lamellar aggregates of the light-harvesting chlorophyll a/b protein complex of photosystem II with long-range chiral order and structural flexibility. Anal. Biochem. 250 169-75. 

I.J. Ryrie and N. Fuad, Membrane adhesion in reconstituted proteoliposomes containing the light-harvesting chlorophyll a/b-protein complex the role of charged surface groups, Arch. Biochem. Biophys. 214 (1982) 475-488. 

K.S. Kan and J.P. Thomber, Hie light-harvesting chlorophyll a/b-protein complex of Chlamydomonas reinhardii, Plant Physiol. 57 (1976)47-52. 

In contrast to LHCI, the light-harvesting chlorophyll a/b-antennae complex of photosystem II (LHCII) is the major component of the particles on the complementary protoplasmic fracture face of appressed membranes (PFs) (Simpson, 1979, Olive et al., 1979), and does not appear to be a significant component of the reaction centre EFs particles, although this is disputed. The LHCII in PFs particles is, nevertheless, in contact with the reaction centre particles and may provide a pathway for excitation energy transfer between several photosystem II reaction centres. 

Dunahay, T. and L A. Staehelin. 1986. Isolation and characterization of a new minor chlorophyll a/b-protein complex (CP24) from spinach. Plant Physiol. 80,429 134. 

The integral membrane chlorophyll a/b antenna complex from higher plants forms two-dimensional crystals, which have been analyzed using electron diffraction and electron microscopy, and also by three dimensional crystals by X-ray crystallography." ... 

Harrison MA and Melis A. (1992). Organization and stability of polypeptides associated with the chlorophyll a-b light-harvesting complex of photosystem-II. Plant Cell Physiol. 33, 627-637. 

Sukenik A, Bennett J, Falkowski PG. (1988). Changes in the abundance of individual apoproteins of light-harvesting chlorophyll a/b-protein complexes of photosystem I and II with growth irradiance in the marine chlorophyte Dunaliella tertiolecta. Biochim. Biophys. Acta 932, 206-215. 

Fig. 2. (A) A hydropathy plot of the amino-acid sequence of pea LHC II. (B) A sketch of the amino-acid sequence of the LHC II from Lemna gibba (an aquatic monocot). (A) from R Bargi, F Suter and H Zuber (1987) Arrangement of the light-harvesting Chlorophyll a/b protein complex in the thylakoid membrane. Biochim Biophys Acta 890 348 (B) from GA Karlin-Neumann, BD Kohorn, JP Thornber and EM Tobin (1985) A chlorophyll a/b-protein encoded by a gene containing an Intron with characteristics of a (rans-posable element. J Mol AppI Genet. 3 58.

JE Mullet, JJ Burke and CJ Arntzen (1980) Chlorophyll proteins of photosystem I. Plant Physiol 65 814-822 E Lam, W Ortiz, S Mayfield and R Malkin (1984) Isolation and characterization of a light-harvesting chlorophyll a/b protein complex associated with photosystem I. Plant Physiol 74 650-655 E Lam, W Ortiz and R Malkin (1984) Chlorophyll alb proteins of photosystem I. FEBS Lett 168 10-14 H Michel (1982) 3-dimensional crystals of a membrane protein complex. The photosynthetic reaction center from Rhodopseudomonas viridis. J Mol Biol 158 567-572... 

Note that the ring planes of the chlorophyll molecules in Fig. 5 (A) were shown oriented mostly perpendicular to the membrane plane. This orientation appears to be similar to that in the PS-II chlorophyll a/b protein (LHC II), as determined by Kiihlbrandt and Wang and predicted by Haworth, Tapie, Amtzen and Breton on the basis of circular-dichroism measurements. The center-to-center distances between the porphyrin planes of the CA-Chl a molecules in the PS-I reaction center range from 8 to 15 A, which are comparable to those in PS tf and in the BChl-a protein complex as measured by Mathews, Fenna, Bolognesi and Olson (see Chapter 8, Section V). Some of the closely spaced chlorophyll-a molecules appear to form chains mnning between the stroma and lumen sides of photosystem I. Between 12 and 16 P-carotene molecules are thought to be present in the PS-I reaction center, but they have not been identified by X-ray crystallographic measurements. 

Paulsen H, Riimler U and Rudiger W (1990) Reconstitution of pigment-containing complexes from light-harvesting chlorophyll a/b-binding protein overexpressed in E. coli. Planta 181 204-211... 

Ruban AV, Phillip D, Young AJ and Horton P (1997) Carotenoid dependent oligomerization ofthe major chlorophyll a/b light harvesting complex of Photosystem II of plants. Biochemistry 36 7855-7859... 

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