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Chloroform, binding affinity

Chloroform-methanol soluble protein with low molecular mass ( 15 kDa) showing binding affinity to GM3 [51]. [Pg.1869]

Crude chloroform-methanol-water (30 60 8, v/v) extracts of immunostainedTLC bands were analyzed without further purification by nanoelectrospray low-energy mass spectrometry. The authors showed that this effective PLC/MS-joined procedure offers a wide range of applications for any carbohydrate-binding agents such as bacterial toxins, plant lectins, and others. Phenyl-boronic acid (PBA) immobilized on stationary support phases can be put to similar applications. This technology, named boronate affinity chromatography (BAC), consists of a chemical reaction of 1,2- and 1,3-diols with the bonded-phase PBA to form a stable... [Pg.209]

The general affinity of guanidinium cations for oxoanions and the spatial direction of the two NH-protons makes the bicycloguanidinium core presented in the preceding chapters also a useful building block for the design of nucleotide receptors [96]. Besides carboxylates (see Sect. 2), bis(naphthoyl) host 12a binds several phophoesters in chloroform, for example, l,r-binaphthyl-2,2 -diyl phosphate the complex of the (S)-enantiomer is shown in formula 64. Still, despite the chiral nature of 12a, no enatioselectivity was observed [97]. [Pg.120]

Fig. 2.1 Chelate effects in high affinity artificial complexes (1) an artificial siderophore with K =l0 M (2) a ionophore binding Cs" ions in chloroform with AC = 90 kj/mol (3) an azacrown ether and triphosphate residue (as in ATP) as guest, with /f=10" M" (only 7 out of the possible 10 to 12 charge-charge... Fig. 2.1 Chelate effects in high affinity artificial complexes (1) an artificial siderophore with K =l0 M (2) a ionophore binding Cs" ions in chloroform with AC = 90 kj/mol (3) an azacrown ether and triphosphate residue (as in ATP) as guest, with /f=10" M" (only 7 out of the possible 10 to 12 charge-charge...

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See also in sourсe #XX -- [ Pg.53 ]




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Binding affinity

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