Chloramphenicol detection chromatographic methods

Agababa et al. (1996) developed a simple method for TLC determination of sulfamethoxazole, trimethoprim, and impurities of sulfanilamide and sulfanilic acid using a chloroform-n-heptane-ethanol (3 3 3) mobile phase. The zones were quantified by scanning densitometry in the reflectance/absorbance mode at 260 nm. Abjean (1997) described a method for multiclass and multiresidue qualitative detection of chloramphenicol, nitrofuran, and sulfonamide residues in animal muscle. The drugs were extracted from 1 gram of tissue with 2 ml of ethanol acetate and purified by silica solid-phase extraction. After elution of the cartridge, the collection solution was evaporated the residue was dissolved in methanol and chromatographed on silica gel 60-HPTLC plates. 

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