Chiral eluant HPLC

Assay of the reaction mixture. The samples were then resuspended in 1.5 mL isopropanol and assayed to determine both the yield and ee by chiral normal phase high-performance liquid chromatography (HPLC). A 250 mm x 4.6 mm Chiralpak AD-H column was used with an eluant of 95 5 heptane/ethanol, a flow rate of 3 mL min a temperature of 10 °C and a detection wavelength of 210 nm. 

Figure 4. Separation by HPLC of the D,L amino acid enantiomers using a chiral reagent. The D,L order Is obtained when L-Prollne Is added to the carrier In the presence of cupric sulfate. The enantiomer resolution Is reversed using D-Prollne. No optical resolution Is obtained when the carrier chlralty Is removed, l.e. with the use of the racemic D,L eluant (17).

The HPLC characterization of the major 3,4-dihydro-2i/-l,2,4-benzothiadiazine 1,1-dioxide diuretics has been discussed in detail <85JC395>, and their racemic mixtures have been resolved into the pure enantiomers by liquid chromatography on a variety of stationary phases including chiral polyacrylamides (1 1 toluene-dioxan as eluant) <85JPS438>, (R)(-l-)-camphanic acid and V-(IS)-... 

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