Chimeric kinase activity sensors

Figure 1.6 Chimeric kinase activity sensors, (a) Schematic of a chimeric sensor (b) schematic of kinase iiiustrating docking groove relative to ATP binding site (c) preparation of ERK activity sensor and (d) sequence of p38 activity sensor.

Alternatively, following stimulation, the kinase of interest can be immunode-pleted using an antibody specific for the desired kinase (Figure 1.7c). In this case, residual kinase activity in the depleted lysate can be attributed to sensor cross talk. The chimeric ERK sensor was found to be selective for ERK in this manner. 

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