Chimeric experiments

The ability of a receptor channel to conduct ions, which is measured as the conductance (the reciprocal of resistance) of the channel, depends on TM2. The experiments that showed this were based on the observation that receptors made of Torpedo a, p, v, and 8 subunits had a different conductance from receptors made of Torpedo a, p, y, and calf 6 subunits. When chimeric 8 subunits in which parts of the Torpedo sequences were replaced by the corresponding calf sequence, were... 

Initial studies of brain delivery based on the chimeric peptide strategy used the absorptive-mediated uptake of cationized albumin which was chemically coupled to the opioid peptide P-endorphin [80] or its metabohcaUy stabilized analogue [D-Ala ]P-endorphin. Tracer experiments in which the chimeric peptide was labelled in the endorphin moiety provided evidence of internalization by isolated brain capillaries and transport into brain tissue in vivo [81]. 

Examples of specialized molecular therapeutics that have been developed from discoveries made using expression profiling experiments include chimeric human/murine antibodies for the treatment of various cancers. Ritux-imab (Rituxan Genentech Inc. South San Francisco, CA), an anti-CD20 antibody in use since 1997, was the first chimeric monoclonal antibody to... 

---