Chemostats steady state conditions, 75-6 nutrient

Our second experimental system consisted of two chemostats linked in series. In the first vessel the bacterial prey was allowed to come to steady state and then fed into the second stage vessel which contained the amoebae. As the limiting nutrient source for the bacteria is virtually exhausted under steady state conditions, it was assumed that no further growth of prey occurred in the second vessel. In the second vessel the equation of balance for the predator is ... 

In the case of mcZ-PHAs, a Swiss group reports the continuous, growth-associated production of poly(3-hydroxyalkanoate-co-3-hydroxyalkenoates) in one-stage chemostat cultures of Pseudomonas putida ATCC 29147 in a single CSTR. The applied substrates encompassed 5-phenylvalerate, octanoate and 10-undecenoate. Multiple nutrient limited growth conditions were chosen at a dilution rate of D = 0.1 h . Different mixtures of the substrates in the feed resulted in the formation of copolyesters with varying compositions and different amounts of aromatic and unsaturated side chains that make the products accessible for further modification. Based on the results, the authors conclude that the steady state conditions in a continuous culture provide a strategy especially suited for the production of tailored PHA copolymers [117]. 

Chemostat A bioreactor in which steady-state growth of microorganisms is maintained over prolonged periods of time under sterile conditions by providing the cells with constant input of nutrients and continuously removing effluent with cells as output. 

---