Chemoenzymatic processes integration

An Integrated Approach to Developing Chemoenzymatic Processes at the Industrial Scale... 

A chemoenzymatic process for the preparation of D- and L-t/ireo-phenylisoserine and derivatives thereof has been developed by Celgene. This process integrates an Erlenmeyer condensation as an initial chemical step which produces racemic threo... 

A further exciting development in the field of chemoenzymatic one-pot synthesis is the integration of artificial metalloproteins (which can then be regarded as the chemocatalytic component ) in such processes. Such a concept was successfully realized by HoUmann, Turner, and Ward et al. in the combination of an artificial transfer hydrogenase with various redox biocatalysts, comprising NADH-, FAD, and heme-dependent enzymes [47]. A selected example is shown in Scheme 19.18. Therein, readily available L-lysine is oxidized by an L-amino acid oxidase toward Al-piperidine<arboxylic acid (52), which is then reduced by the iridium complex-containing metalloprotein to racemic pipecohc acid (rac-53). 

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