Chemical synthesis solubility problem

The detection of products derived from the N-oxygenation of C=N functionalities presents many problems, which illustrate difficulties that are associated with the isolation, identification and quantification of small amounts of water-soluble metabolites. Spectrophotometric methods19 as well as differential pulse polarographic techniques20 previously used to determine oximes, nitrones and N-oxides frequently lack sensitivity and/or specificity. Improved analytical methods for the quantification of these N-oxy compounds include chromatographic techniques taking into account the chemical peculiarities of the individual N-oxygenated C=N functionalities. These procedures usually require the chemical synthesis of authentic material for comparison with data obtained with the isolated metabolites, and also for the construction of calibration curves. 

The large difference in solubility of Ba and Fe sources and the consequent problems in the wet chemical synthesis of Ba-Fe compounds prompted Chhabra et al. [215] to use a new and modified method of synthesis through microemulsions. In this method, the dispersed phase was anhydrous ethanol while the continuous phase was isooctane. 

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