Chemical modifications cobalt enzyme

The ionizable ligand, EH2, is assigned to Glu270, since chemical modification of this residue with CMC (1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide) decreases the binding affinity of carboxypeptidase A for zinc and lead by more than 60- and 200-fold, respectively. A bridging interaction between the Glu270-coordinated zinc hydroxide is implicated by the ability of Zn and Pb to induce a marked increase in the visible absorption spectrum of the cobalt enzyme within 20 ms of mixing 10 " M Zn or Pb with 10 " M cobalt enzyme. 

Figure 3.11 shows the chemical deposition process of GOx enzyme assembled 2-mercaptoethanol on gold that was previously modified by cobalt tetracarboxylic acid chloride phthalocyanine self-assembled monolayer gold. Electrochemical biosensor was introduced in the following steps pretreatment hydrolysis functionalized surface -> enzyme modification. Shervedani and his coworkers reported a similar... 

---