Chemical cleavage protocol

Two basic protocols for DNA sequencing are commonly adapted in laboratories the chemical cleavage method (Maxam and Gilbert, 1977) and the enzymatic chain termination or dideoxy method (Sanger et al, 1977). Of the two approaches, the Sanger dideoxy... 

Peptide hydrolysis, complete hydrolysis of peptides and proteins for amino acid analysis or the production of individual amino acids from the peptide or protein hydrolysate. For this purpose, numerous chemical and enzymatic protocols are known, but none of these procedures alone is fully satisfactory. Besides hydrolysis with 6 M hydrochloric acid at 120 °C for 12 h, or with dilute alkali (2-4 M NaOH) at 100 °C for 4-8 h, mixtures of peptidases can also be used for complete peptide hydrolysis. Restricted or limited peptide hydrolysis ( peptide cleavage) is important for - sequence analysis and peptide mapping. 

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