Chemical carcinogenesis acetylaminofluorene

Human exposures to chemicals found in certain foods or environmental pollutants are linked to a higher risk for cancer. It is known that the initial step in chemical carcinogenesis is the covalent attachment of the chemical to DNA to produce DNA adducts. The structure identification of these DNA adducts is very important in cancer research. CEC-ESI-MS with shealth liquid interface has been demonstrated as a good tool for the analysis of DNA adducts as well as nucleosides with styrene oxide by Vouros and co-authors [24, 25]. Two common DNA adducts, acetylaminofluorene deoxyguanosine (AAF-dG) and an isomer of anti-benzo[g]chrysene 11,12-dihydrodiol 13,14-epoxide, were employed as model compounds. CEC separation was performed on a Cig silica gel-packed column and used a mixture of 60% acetonitrile and 5 mM ammonium acetate as the mobile phase. Figure 10 shows the extracted ion electrochromatograms and mass spectra of AAF-dG and benzo[g]chrysene-dG [25]. 

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