Chanoclavine cyclase

The oxidation and cyclization of chanoclavine is dependent on the cultivation conditions. The enzyme, chanoclavine cyclase, reponsible for this biochemical reaction, is a membrane bound enzyme and is thus influenced by membrane-affecting agents. This was studied with Claviceps purpurea mutant... 

The chanoclavine cyclase activity is dependent on NADH or NADPH, Mg, and ATP (10). The scheme we propose here is consistent with an NAD(P)H requirement for the third enzyme (reductase). Also, we expect the oxidized form, NADP or NAD, to oxidize 16 to aldehyde 17. Furthermore, and as stated above, it is possible that NAD(P)H is involved in the oxidation and reduction reactions to generate iminium ion 21. However, it is not obvious why there is a requirement for ATP. Clearly, elucidation of the mechanisms underlying the steps in D-ring formation will continue to reveal some interesting biochemistry. 

Dimethylallyl pyrophosphate L-tryptophan dimethylallyltransferase, chanoclavine-I-cyclase (D 21.2) Agroclavine, elymoclavine, lysergic acid I. R ... 

Four enzymes which are involved in the biosynthesis of the ergot alkaloids have now been isolated from Claviceps. The other three are 4-dimethylallyltryptophan synthase , chanoclavine-I cyclase , and agroclavine hydroxylase . ... 

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