Chalfie

Chalfie, M., et al., 1994. Green fluorescent protein as a marker for gene expression. Science 263 802-805. 

The gene of Aequorea GFP was cloned by Prasher et al. (1992), and expressed in E. coli and Caenorhabditis elegans by Chalfie et al. (1994) and in E. coli by Inouye and Tsuji (1994a). The X-ray structure of recombinant GFP was solved by Ormo et al. (1996) and Yang et al. (1996,1997). The protein is in the shape of a cylinder consisting of 11 strands of (3-sheets and an a-helix inside (which contains the chromophore), with short helical segments on the ends of the cylinder. Thus the chromophore is sealed and protected from the outside medium. 

Chalfie, M. (1995). Green fluorescent protein. Photochem. Photobiol. 62 651-656. 

Chalfie, M., and Kain, S. (1998). Green Fluorescent Protein Properties, Applications, and Protocols. Wiley-Liss, New York, NY. 

M Chalfie, AH Neufeld, JA Zadunaisky. (1972). Action of epinephrine and other cyclic AMP-mediated agents on the chloride transport of the frog cornea. Invest Ophthalmol 11 644-650. 

Chalfie M (2009) GFP lighting up life (Nobel lecture). Angew Chem Int Ed Engl 48 5603-5611... 

Li, C. and Chalfie, M. (1986) FMRFamide-like immunoreactivity in C. elegans. Society for Neuroscience Abstracts 12, 246. 

Mechanosensation in Caenorhabditis elegans Robert O Hagan and Marlin Chalfie... 

Aequorea Victoria (/max = 509 nm) and developed for analytical use by Chalfie,61 Shimomura,62 and Tsuen.63... 

Ward TH, Lippincott-Schwartz J. The uses of green fluorescent protein in manunaUan cells. In Green Huorescent Protein. Chalfie M, Kain SR, eds. 2006. Wiley, New York. 

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