Centralbureau voor Schimmelcultures

Two different strains of Saccharomyces cerevisiae were used CBS 8066 (Centralbureau voor Schimmelcultures, Delft, The Netherlands) and TMB 3000 (Division of Applied Microbiology, Lund University, Sweden). TMB 3000 is a strain originally isolated from spent sulfite liquor, and therefore it has a high tolerance to inhibitors. The strains were maintained on agar plates (20 g/L of agar, 20 g/L of soya peptone, 20 g/L of glucose, and 10 g/L of yeast extract). Before the fermentations, inoculum cultures were grown in 250-mL conical E-flasks. The flasks were placed in a shaker bath (30°C, 140 rpm) for 24 h. 

STELLiNG-DEKKER, N. M. (1931). Die Hefesammlungdes Centralbureau voor Schimmelcultures, /. Teil. Die sporogenen Hefen, Amsterdam Verhandel. Koninkl. Akad. Wetenschap., Afd. Natuurkunde. 

DiDDENS, H. A. and LODDER, j. (1942). Die Hefesammlung des Centralbureau voor Schimmelcultures. II. Teil. Zweite halfte N.V. Noord Hollandsche Uitgevers Maatschappij. 

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See also in sourсe #XX -- [ ]

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