Cellulolytic enzymes differentiation

The above example of enzyme analysis together with the results obtained from studies of other systems, point to a great potential of using covalently dyed polysaccharides in combination with fluorogenic glycosides for differentiation of cellulolytic enzymes. This fact is illustrated in Table 1. 

Table 1. Differentiation of Cellulolytic Enzymes According to their Substrate Specificities using Covalently Dyed Hydroxyethyl-cellulose (OBR-HEC) and xylan (RBB-X) and 4-methylumbelliferyl 3-glycosides as Substrates. For abbreviations see the text.

Biely P (1987) Differentiation of glycanases of microbial cellulolytic systems using chromogenic and fluorogenic substrates. In Chaloupka J, Krumphanzl V, (eds) Extracellular enzymes of Microorganisms. Plenum Press New York, p 187... 

Biely P (1990) Artificial substrate for cellulolytic glycanases and their use for the differentiation of Trichoderma enzymes. In Kubicek CP, Eveleigh DE, Esterbauer H, Sten W, Kubicek-Pranz EM (eds) Trichoderma Cellulases. Biochemistry, Genetics, Physiology and Applications. Royal Chem Soc Cambridge p 30... 

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