Cellulases accessibility measurement

Cel/ulomonas fimi Cex cellulase-xylanase /3-Side The complex with 2.4-dinitroplicnyl-2-fluoro-/3-cellobioside shows88 bound 2-fluorocellobiose with C-l covalently a-linked to the OP.l of the nucleophilic residue Glu233. No measurements of the accessibility of Glu233 to solvent are reported. 

Conventionally, cellulase adsorption onto (or accessibility for) cellulose, such as that of T. reesei cellulases onto amorphous/crystalline/lingo-cellulose, has been investigated by Langmuir-type isotherms 20, 34). They measure overall cellulase protein adsorption, assuming a uniform cellulose surface. 

Langmuir-type isotherms, governed by an adsorption constant and a capability No, are widely used to measure the adsorption of cellulase onto cellulose. The parameter No reflects the portion of s ace cellobiosyUattice units accessible for cellulase adsorption, regardless whether or not the adsorption is followed by hydrolysis. In contrast to the static No, is kinetic and measures those productive cellulase adsorptions that can lead to subsequent p-1,4 bond cleavage. 

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