Cellulase carbohydrate content

For comparison some properties of hydrocellulases A, B, and C are presented in Table VI with those of the three cellulases, II, III, and IV, prepared in Nisizawas laboratory (55). The carbohydrate contents appear similar with increasing levels in the order hydrocellulase A < B C and Cellulase IV < III II. The hydrocellulase carbohydrate content was determined as glucose and thus should be reduced slightly for comparison with the cellulase values determined as mannose. In each... 

Adney, W. S., Mohagheghi, A., Thomas, S. R., and Himmel, M. E., Comparison of protein contents of cellulase preparations in a worldwide round-robin assay. In Enzymatic Degradation of Insoluble Carbohydrates, 1995 Vol. 618, pp. 256-271. 

Two extracellular cellulases that act on carboxymethylcellulose have been isolated from the supernatant fluids of cultures of Sporocytophaga myxococcoid.es by gel-filtration and ion-exchange chromatography. More cellulase II (mol. wt. 5.2 X 10 by gel electrophoresis, p/ 4.75, pH optimum 5.5—7.5) was present than cellulase I (mol. wt. 4.6 x 10 , p/7.5, pH optimum 6.5—7.5). Both cellulases have very low contents of carbohydrate, possibly as impurities, and similar amino-acid compositions, and are endo-enzymes. A cell-bound cellulase was also partly purified. 

The sugar content and composition of solubilised carbohydrates of cellulose treated by purified cellulases from Trichoderma reesei have been estimated (see Table 4.3)." ... 

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