Celluclast Cellulase

Although most cellulase enzyme complexes, including Celluclast, contain xylanase activity (data not shown), the amount of released xylose after enzymatic hydrolysis was negligible, especially compared with the amount of released xylose after pretreatment. 

Three enzymatic mixtures with predominantly cellulase and xylanase activity were used for the saccharification tests. To hydrolyse the cellulose, the mixtures Celluclast 1.5L and Novozym 188 from Novo Nordisk were... 

Enzymes were supplied by Novozyme (US Office Franklinton, NC) and included cellulase (Celluclast 1.5 L 48 international filter paper units [IFPU]/mL), (3-glucosidase (Novozym 188 66.8 x 103 IU/mL), and glucoamylase (Novozyme AMG300L). Sugars were purchased from Sigma (St. Louis, MO), and all other chemical and media reagents were from Fisher (Fairview, NJ). 

The hemicellulose-degrading enzymes (Ultraflo L) and the cellulases (Celluclast 1.5L) were kindly donated by Novozymes A/S. Celluclast 1.5L had a filter paper activity of 80 filter paper units/mL, determined according to the procedure of Mandels (8-9). For the present study the most important side activities of Ultraflo L were endo-l,4-P-xylanase, P-xylo-sidase, and a-arabinofuranosidase (10). 

Commercial cellulase and P-glucosidase (Novo Nordisk, Bagvaerd, Denmark) supplied from Novozymes Korea were used. A mixture of Celluclast (80 IU or international filter paper units [IFPU]/mL) and Novozym 188 (792 cellobiase units [CBU]/mL) was used with a ratio of 4 IU of Celluclast/CBU of Novozym to alleviate end-product inhibition by cellobiose. 

The enzyme preparations were provided by Novo Nordisk. Cellulase (Celluclast) possessed 115 filter paper units (FPU)/mL P-glucosidase (Novozym 188) had 570 cellobiose units (CBU)/mL. 

All hydrolysis experiments were conducted with a combination of cellulase (Celluclast) and P-glucosidase (Novozym 188) at a ratio of 1 2... 

Celluclast. [Novo Nordisk] Cellulase enzyme for breakdown of cellulosic material for prod, of fermentable sugar and for municipal cellulosic waste treatment. 

Two commercials enzymes, Celluclast 1.5-L and Novozyme 188 (Sigma, St Louis, MO, USA), were used for enzymatic hydrolysis. Celluclast 1.5-L contained 98.2 FPU/ml of total cellulase P-glucosidase activity of Novozyme-188 was 540 unit/ml. One FPU is defined as the enzyme amount that releases 1 p.mol of glucose equivalents from Whatman no. 1 filter paper in 1 min. One unit of 3-glucosidase activity is defined as the enzyme amount that converts 1 imol of cellubiose to 2 pmol of glucose in 1 min [17],... 

Celluclast 200 L [Novozymes A/S http //www.novozymes.com], Celluclast 200 L Type N [Novozymes A/S http //www.novozymes.com], Cellulase 4000 t[Genencor Int l. http //www.genencor.com], Cellulase AC [Genencor Int l. http //www.genencor.com]-, Cellulase AP [Mitsubishi Int l. http //www.micusa.com, Amano Enzyme USA]... 

Celluclast 2.0 L Type X Celluclast 200 L Celluclast 200 L Type N Cellulase Cellulase 4000 Cellulase AC Cellulase AP Cellulase AP3 Cellulase DS Cellulase L Cellulase TAP Cellulase T-AP6 Cellulase Tr Cone. 

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