Cellobioside hydrolase

In view of the efficient inhibition of a particular crystalline glycan hydrolase by the epoxylbutyl -cellobioside 7 (n = 2), we decided to prepare the deoxy iodo derivative 21 to aid in the X-ray crystallographic analysis. We soon found that, although the alkene 22 was easily available as a direct precursor to our target, the epoxide functionality had to be introduced indirectly using bromo-hydrin 23 technology any direct oxidation of 22 invariably led to some loss of the iodine atom [23]. 

Stachybotrys atra (235). An intracellular enzyme from this fungus, which released glucose as the sole product from / -l,4-oligoglucosides up to CL 11 and was not inhibited by 1,5-gluconolactone (236), is apparently an exo-hydrolase. The / -l,4-glucosyl linkage in p-nitrophenyl-a-cellobioside was also hydrolyzed. 

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