Cellobiose dehydrogenase CDH

Direct electron transfer-a favorite electron route for cellobiose dehydrogenase (CDH) from Trametes villosa. Comparison with CDH from Phanerochaete chrysosporium. Langmuir, 22 (25), 10801-10806. 

Cellobiose, lactose, glucose maltose Cellobiose dehydrogenase (CDH, EC 1.1.99.18)... 

Cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium (0.84 g l in 50 mM ammonium acetate buffer, pH 5) (23,26) was kindly provided by Dr. G. Henriksson and Dr. G. Pettersson (Department of Biochemistry, Uppsala University, Sweden) and horseradish peroxidase (HRP) was purchased from Boehringer-Mannheim, Mannheim, Germany (cat. no. 814407, RZ 3.2-3.3, approx. 1000 U /mg (ABTS)). 

Cellobiose dehydrogenase (CDH) preferentially oxidizes cellobiose, the product of cellulose hydrolysis by cellulases. A variety of substrates can be used as electron acceptors, including quinones, transition metals, phenoxyl radicals, and O2 (Figure 1C, 14). A possible secondary reaction is the production of Fenton reagent resulting in the hydroxyl radical. 

Cellobiose dehydrogenase (CDH) is another enzyme that has been explored for its potential to undergo DET. CDH consists of an FAD-dependent dehydrogenase domain, which is responsible for the oxidation of substrates, and an additional cytochrome heme-containing domain. Following the 2e oxidation of a substrate at the FAD-dehydrogenase domain, an electron mediator (such as hydroquinone) can be employed to transfer electrons between the FAD-dehydrogenase domain and an electrode. In a similar fashion to PQQ-AldDH and... 

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