Cellobiase measurement

Cellobiase can be measured by following the glucose production from cellobiose and cellodextrins, the saligenin from salicin, and the p-nitrophenol formation from its / -glucoside (2). 

During purification procedures cellobiase activity was monitored by measuring nitrophenol (at A42onm) release for p-nitrophenyl-/ -D-glucoside (JO). Kinetic studies and enzyme characterization were carried out using / -D-cellobiose as substrate with the product, glucose, measured with a Beckman Glucose Analyzer (JO). Assay conditions were pH 4.8 and 50°C. 

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