Cell viability, poly hydrogels

Jabbari Esmaiel, and Karbasi Saeed. Swelling behavior and cell viability of dehydrother-mally crosslinked poly(vinyl alcohol) hydrogel grafted with N-vinyl pyrrolidone or acrylic acid using y-radiation. J. Appl. Polym. Sci. 91 (2004) 2862-2868. 

This photocurable ink design can be extended to many polymeric systems, including poly(2-hydroxyethyl methacrylate), a well-characterized, biocompatible hydrogel material [30]. We therefore envision many opportunities to further tailor the scaffold chemistry, mechanical properties, and structure to improve cell viability on these novel constructs. 

Redox-initiated polymerization using ammonium persulfate (APS)/N,N,N, N -tetramethylethylenediamine (TEMED) or APS/ascorbic acid has been used to encapsulate cells in poly(ethylene glycol) (PEG), oligo(PEG fumarate) (OPE), poly (lactide-co-ethylene oxide-co-fumarate) (PLEOF), chitosan derivatives, or carboxybetaine hydrogels [6-11]. In these systems, an increase of initiator concentration led a decrease in the gelling time, however, it also affected the cell viability. Therefore, low cytotoxic free radical polymerization is needed to use this method for biomedical applications. 

To mimic the macromolecular-based ECM in biological tissue, the cell adhesion and proliferation properties of hydrogels are critical parameters. However, various hydrogels that originate from natural resources, such as alginate [87], chitosan [88, 89], and hyaluronic acid [90], and that are synthetically created, such as poly (7V-isopropylacrylamide) (PNIPAAm) [91], PEO [92], PVA [93], and poly(ethylene glycol) (PEG) [94], show a poor cellular viability without modification with cell adhesive proteins or peptides, such as collagen, laminin, fibronectin, and the RGD (Arg-Gly-Asp) sequence. 

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