Cell separation hematocrit

Blood cells are separated from blood (hematocrit 40%) by microfiltration, using hollow-fiber membranes with an inside diameter of 300 pm and a length of 20 cm. The average flow rate of blood is 5.5 cm s T Estimate the filtrate flux. 

A preliminary investigation was made of the toxicity of the graft copolymer emulsion using human erythrocytes as a model cell line. First of all the toxicity of an emulsion prepared from 5% HEMA and 1% alginate in phosphate buffered saline was estimated by examination of a mixture with 10% hematocrit after 1 h storage. No lysis could be observed and the cells could be separated and disrupted in distilled water. We then encapsulated 10% hematocrit in the same emulsion and stored the capsules in isotonic saline for one week. The cells were recovered by disruption of the capsules with citrate and were perceived as healthy as per the simple tests outlined above. 

Interfacial Electrokinetic Flow, Fig. 14 Sequence of recirculation. The final plasma effluent above contains images showing the separation of red blood cells from a hematocrit less than 0.003 % (After Yeo et al. [19]) blood plasma via the secondary meridional bulk liquid... 

Based on a similar principle, Yang et al. [9] have developed a microfluidic device for continuous, real-time blood plasma separation from whole blood. The device is composed of a blood inlet, a bifurcating region which leads to a purified plasma outlet, and a concentrated blood cell outlet. They have precisely designed flow rate ratios at each bifurcation so that no blood cells (erythrocytes, leukocytes, or platelets) flow into the side channels, while blood plasma is skimmed into the purified plasma collection channels. The experimentally determined plasma selectivity with respect to blood hematocrit level was almost 100 % regardless of the inlet hematocrit (Fig. 7a). The total plasma separation volume percent varied from 15 % to 25 % with increasing inlet hematocrit (Fig. 7b). 

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