Cell preparation for biomechanistic studies

Glutaraldehyde-osmium tetroxide and critical point drying 

Carnoy s fixative (60% absolute ethanol, 30% chloroform, 10% glacial acetic acid) 

Loss of lipid ester peak (1756-1722cm ) due to exposure to acetone and ethanoF  

Reduction in intensity of 1500-1000 cm Region and less resolved lipid ester signal (1756-1722 cm-Y  

Loss of features in 1253-1127 cm region Less intense bands at 1612cm- and 1180cm- compared with formalin 

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