Cell disruption autolysis

The recovery of intracellular proteins involves distinct cell disruption procedures, depending on the cell characteristics. For the processing of animal cells, which do not have a cellular wall, mild and moderate techniques are commonly used. Mild techniques include cell lysis by enzymatic digestion, chemical solubilization or autolysis and the use of manual homogenizers and grinders, whereas the moderate techniques involve blade homogenizers and abrasive grinding. 

A common problem associated with rupture of yeast cells and protein extraction is proteolysis. Yeast cells contain a full complement of intracellular proteolytic enzymes which may be liberated after the cells are broken either by autolysis or by mechanical disruption. These liberated proteolytic enzymes, unless inactivated during the isolation and purification of yeast proteins, hydrolyze the proteins causing poor yields of intact protein (55, 69,70). 

Cell lysis or autolysis Osmotic disruption of cell membrane under high osmotic pressure (usually 20% sucrose) or detergent. Autolysis (e.g. yeast) is generally carried out in the presence of toluene. 

It has long been known that bacterial luminescence is intimately associated with the cells (165), and there is yet no certain evidence that luminescence has been observed apart from the living bacteria. Various means of disrupting cell structure—cytolytic agents, osmolysis, autolysis, mechanical grinding, supersonic vibration—apparently destroy the capacity for luminescence (138), although the activity of certain enzyme systems,... 

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