Cation chromatography temperature effect

The isolation, purification, and characterization of oc-D-mannosidase from cultures of Aspergillus flavus has been described.The enzyme (pH optimum 4.2-4.5) was isolated from the growth medium of the mold at the beginning of the stationary phase of growth and partly purified by ion-exchange chromatography. The effect of temperature and cations on the activity of the enzyme and the effect of pH on its stability were examined for 4-nitrophenyl a-D-mannopyranoside 1.4 mM, the value of the sedimentation constant is. y o = 4.5 S, and the partial specific volume q = 0.725 cm g" ). 

F12. Fliickiger, R., and Woodtli, T., Effect of temperature on quantifying glycated (glycosylated) hemoglobin by cation-exchange chromatography. Clin. Chem. 31, 114-117... 

Figure 6 Effect of chiraiity of the mobiie phase additives on the separation of d- and i-amino acid enantiomers by iigand-exchange chromatography. Coiumn DC 4a cation-exchange resin (12 x 0.2 cm). Mobiie phase sodium acetate buffer (0.05moii pH 5.5) containing 4xi0 moii CUSO4 and 8 X 10 moii proiine. Coiumn temperature 75°C. Fiow rate 10 mi h h (Reprinted (abstracted/experted) with permission from Hare PE and Gii-Av E (1979) Science 204 1227 American Association for the Advancement of Science.)...

When columns filled with NaY and also with CdY zeolite were used with carbon dioxide as carrier gas, a considerable decrease in specific retention volumes of hydrocarbon gases was obtained, especially at low temperatures typical of carbon dioxide adsorption on zeolites, screening of cations and partial deactivation of the adsorbents. Use of carbon dioxide as carrier gas improves the separating ability of zeolites without complete suppression of the cation effects on the separation and widens the range of zeolite applications in gas chromatography [109]. 

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