Catalytic response to glucose

Electrochemical Behavior, Catalytic Response to Glucose, and Selectivity to Pharmaceutical Drugs... 

Effect of pH. The relationship between the catalytic current of a PPy-GOD film (2000 A) and the pH of the solution was recorded in the pH range of 3-11. When the pH was less than 3, no catalytic current was observed, but the current was increased by increasing the pH from 3 to 7. When pH was more than 7.5, the current decreased. The PPy-GOD film electrode showed a good catalytic response to glucose in the solutions of pH ranging between 6 and 8. 

Polypyrrole thin film doped with glucose oxidase (PPy-GOD) has been prepared on a glassy carbon electrode by the electrochemical polymerization of the pyrrole monomer in the solution of glucose oxidase enzyme in the absence of other supporting electrolytes. The cyclic voltammetry of the PPy-GOD film electrode shows electrochemical activity which is mainly due to the redox reaction of the PPy in the film. Both in situ Raman and in situ UV-visible spectroscopic results also show the formation of the PPy film, which can be oxidized and reduced by the application of the redox potential. A good catalytic response to the glucose and an electrochemical selectivity to some hydrophilic pharmaceutical drugs are seen at the PPy-GOD film electrode. 

Figure 2.26 (a) Scheme for an electrostatically self-assembled multilayer (PAHOs)4(Apo-COx)3(COx) electrode, (b) Catalytic current response to p-d-glucose concentration for self-assembled nanostructured thin films of PAH-Os/COx/(PAH-Os/(ApoGOx)3 and (PAH-Os/ApoCOx)3/PAH-Os/COx, where ApoCOx is FAD-free glucose oxidase. Taken from Ref [219]. 

Figure 6. Catalytic response of PPy-GOD film to glucose, (a) Current response at PPy-GOD film electrodes with successive additions of 2.5 mM glucose solution (1) 80 A film and (2) 2000 A film, in PB buffer (pH 7.4) at 1.0 V. (b) Calibration curves. 1 80 A film, 2 2000 A film.

Wallberg-Hendrilcsson, H., and Holloszy, J. O. 1985. Activation of glucose transport in diabetic muscle Responses to contraction and insulin. Am. J. Piii sioi 249, C2.33-C237, Weber, J., and Senior, A. E, (1997, Catalytic mechanism of IvATPase. BiocJjj m. Bic p ys- Ada 1319,19-58. 

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