Catalysis via Protease

It has been noted [6] that despite their favorable catalytic properties, proteases are not necessarily ideal catalysts for peptide synthesis. Hydrolysis is always a concern which prevents the formation of high-molecular-weight polymers. A number of strategies have been designed to optimize reactions, such as improved proteases, substrate modification, modification of the physical state of reactants, 

In a non-polypeptide example, Gu, et al. [39] attempted to use four proteases (chymotrypsin, trypsin, subtilisin, and papain) to polymerize water-soluble polyamides from dimethyl adipate and diethylene triamine. However, only oligoamides were obtained. 

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