Cassette dosing advantage

Cassette dosing or N into 1 dosing was one of the first techniques used to enhance the throughput of ADME/PK studies. It has the advantage of reducing the number of animals used, and increasing the number of compounds that can be... 

Typically, fewer than five compounds are cassette-dosed to animals with one of the compounds as the benchmark. The advantage of cassette-dosing is quite obvious it can process more compounds at a time and reduce the number of animals needed for an assay. However, the PK exposure of testing compounds may be compromised due to the potential of systemic drug-drug interactions. ... 

Sample pooling is also used in a process called cassette assay in which samples are pooled from multiple (typically 5 or 6) dosing experiments.24-83 87-88 Hsieh et al.89 showed that one could pool the plasma from six NCEs into one sample per time point to reduce sample assay time. Kuo et al.88 used a similar sample pooling approach for NCEs dosed into rats. The advantage is that cassette assay requires fewer samples. Two disadvantages are the need to dilute samples and the difficult set-up. 

Dance DR, Lester SA, Carlsson GA, Sandborg M, Persliden J, The use of carbon fibre material in radiographic cassettes, estimation of the dose and contrast advantages, Br J Radiology, 70(832), 383-390, 1997. 

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