Japan case report form

Compactin (a gift of Dr. A. Endo, Tokyo Noko University, Japan) was converted to the acid form [3] and stored as 1 mM solution in multiple aliquots at -20 °C until used. Initial experiments were carried out with a shooty line of tobacco (Nicotiana tabacum cv Xanthi) crown gall tissue in which [9R]Z was the major endogenous cytokinin. The tissue (10 g) was incubated in liquid medium (10 ml) for 5 h in the absence or presence of compactin (5 /xM) on a shaker (80 rpm, 22 ° C, under fluorescent light). At the end of this preincubation period 4.5 /xCi of DL-[2- H]-MVA lactone (1.28 Ci/mmol Amersham, UK) was added to each flask. Following further incubation for 10 h the tissues were removed, extracted and purified as previously reported [19]. The uptake of [ H]-M VA was 59 and 56.7% by control and treated tissues, respectively. In both cases about 56% of radioactivity taken up was extracted, and more than 90% of extracted radioactivity was not retained on cellulose phosphate columns. The basic fractions and the nucletoide-derived. 

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