Cascade Blue hydrazide

Figure 9.40 Cascade Blue hydrazide can be used to modify aldehyde-containing molecules to form hydrazone bonds.

Aldehyde/Ketone-Reactive Cascade Blue Hydrazide... 

This fluorophore has an excitation maximum at 400 nm and an emission maximum at 420 nm. The extinction coefficient of the molecule in aqueous solution at pH 7 is about 31,000M 1cm 1. Cascade Blue hydrazide and Lucifer Yellow derivatives can be excited simultaneously by light of less than 400 nm, resulting in two-color detection at 420 and 530nm. 

Cascade Blue hydrazide is soluble in aqueous solution, and it should be stable for awhile if protected from light. A concentrated stock solution of the reagent may be prepared in water and an aliquot added to a buffered reaction medium to facilitate the transfer of small quantities. For aqueous reactions, a pH range of 5-9 will result in efficient hydrazone formation. 

Cascade Blue Hydrazide Aldehyde Containing Molecule Hydrazone Bond Formation... 

Green 488 carboxylic acid, Cascade Blue hydrazide, FQ-labeled aspartic acid, CBQCA-labeled serine and tyrosine, GFP, TAMRA-labeled 20-mer oligonucleotide, and fluorescently labeled PS particles see Figure 5.20). This focusing effect leads to a pre-concentration factor of 10,000 or greater (from 8 nM to 90 dM for Oregon Green) [597]. 

Cascade Blue acetyl azide trisodium salt Cascade Blue hydrazide trisodium salt... 

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