Cartridges, holders

Because they are sold without end fittings, cartridge-type columns are cheaper than conventional columns, although the initial outlay on the cartridge holder has to be considered. 

In general two different types of HPLC columns are available. Prepacked columns are those with metal casings with threads at both ends onto which capillary connections are attached. The cartridge HPLC columns are cheaper and are used with cartridge holders. As the cartridge is fitted with a groove for the holding device, no threads are necessary and the connection pieces can be reused. 

C-18 Cartridge (4 /xm particle size) in a Radial Compression Cartridge Holder solvent, 75% H2O - 25% methanol. Ninety-nine percent of the C activity had the same retention time as reference sulfamethazine when chromatographed using HPLC-1 conditions as described below. The synthesis and purification of N -acetylsulfamethazine has been reported (5). 

Inc. System (two model 6000A pumps model 440 UV detector with a 254 filter model 680 gradient controller model 746 data module and a RCM 8 X 10 cm Radial Compression Cartridge Holder) was used for high pressure liquid chromatography. Injections were made with a model 7125 Rheodyne injector. 

Figure A. Column end-assembly configured for mlcrovolcammecric electrochemical detector AE, auxiliary electrode CA, cartridge holder CH, column holder CM, Column EH, electrode holder FR, frit MM, micromanipulator PP, piezoelectric positioner RE, reference electrode SC screw cap WE, working electrode. (Reproduced from ref. 11. Copyright 1988 American Chemical Society.)...

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