Carboxypeptidase Catalytic antibody

Nitrogenase Methan monooxigenase Fumarase Carboxypeptidase Adenosine deaminase Phosphotriesterase Carbonic anhydrase Catalytic antibodies... 

While no attempts were made to compare the binding capacity of matrices, enzyme immobilized on either support were shown to exhibit full catalytic activity, improvement in stability, and no alteration in Km, Vmax and Kj values. The preparation obtained by direct covalent coupling of carboxypeptidase to support however exhibited relatively lower activity. It was also observed that orientation of the antibody on protein A supports may not after all enhance its enzyme binding activity. The author reasoned that even the most accessible antibody molecule may not bind to more than one enzyme molecule due to the large dimensions of the latter. 

In my own laboratory at Tel-Aviv University we have been testing the effects of different monoclonal antibodies on the activity of carboxypeptidase A [31]. We have found some monoclonal antibodies that inhibit the esterase and peptidase activities of the enzyme, some that inhibit the esterase but not the peptidase activity, others that block the peptidase but not the esterase activity, and yet others that bind to the enzyme but do not markedly affect either of its catalytic activities. Some of the monoclonal antibodies seem to modify the conformation of carboxypeptidase A, thus greatly altering the kinetic parameters of the enzyme. 

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