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Carboxyl-Reactive Biotinylation Agents

Iodoacetyl-LC-biotin has been used to localize the SH thiol of myosin by use of an avidin-biotin complex visualized by electron microscopy (Sutoh et al., 1984) and to determine the spatial relationship between SHj and the actin binding site on the myosin subfragment-1 surface (Yamamoto et al., 1984). [Pg.525]

Dissolve iodoacetyl-LC-biotin (Thermo Fisher) in DMF at a concentration of 4 mM (2 mg/ml). Protect from light. [Pg.525]

Remove excess reactants and reaction by-products by dialysis or gel filtration using a desalting resin. [Pg.525]

See other pages where Carboxyl-Reactive Biotinylation Agents is mentioned: [Pg.525]    [Pg.525]    [Pg.527]    [Pg.529]    [Pg.410]    [Pg.390]    [Pg.525]    [Pg.525]    [Pg.527]    [Pg.529]    [Pg.410]    [Pg.390]    [Pg.451]   


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Biotinylated

Carboxylates. reactivity

Reactivators agents

Reactivity carboxylic

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