CARBOXY-2,7-DICHLOROFLUORESCEIN DIACETATE

CA Index Name Spiro[isobenzofuran-l(3//),9 -[9//] xanthene]-5-carboxylic acid, 3, 6 -bis(acetyloxy)-2, 7 -dichloro-3-oxo- 

Other Names 5-Carboxy-2, 7 -dichlorofluorescein diacetate Carboxy-DCFDA 5-CDCF DA Merck Index Number Not listed Chemical/Dye Class Xanthene Molecular Formula C25H14CI2O9 

Physical Form Light brown solid Off-white solid 

Solubility Insoluble in water soluble acetonitrile, chloroform, A,A-dimethylformamide, dimethyl sulfoxide, methanol 

Industrial Applications Not reported Safety/Toxicity Metabolic toxicity  

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Material required Stock solution of 25 mM H2DCFDA, carboxy-2, 7 -dichlorofluorescein diacetate (Sigma Co) dissolved in dimethyl sulphoxide (DMSO). Confocal Microscope BIORAD 1024, Laser KrAr with inverted microscope Nikon TMD 300. 

Percy, M. E. Zhao, Y. Lukiw, W J. Metal-sulfate induced generation of ROS in human brain cells detection using an isomeric mixture of 5-and 6-carboxy-2, 7 -dichlorofluorescein diacetate... 

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