Carbonic anhydrase limiting factor

The importance of zinc may extend even further as the processes that control carbon uptake by marine phytoplankton are important in the carbon cycle, and the zinc enzyme carbonic anhydrase could be a limiting factor. The concentration of zinc available to some marine phytoplankton has... 

An enzyme electrode is basically a dense package of dialyzer, enzyme reactor, and electrode (detector). Enzymes introduce analytical selectivity due to the specificity of the signal-producing interaction of the enzyme with the analyte. They enhance the equihbrium formation of chemical reactions. For example, splitting of H2O2 is accelerated by a factor of 3 x 10 in the presence of catalase. Turnover numbers can be as fast as 6 X 10 s (carbonic anhydrase) where cat/ m approaches the diffusion limited value of 10 s ... 

A.24.1 kcatIKM. Chymotrypsin = 2 x 10" Carbonic anhydrase = 7.5 x 10 Catalase = 3.89 x 10 Penicillinase = 4 x 10 Lysozyme = 8.33 X 10". Diffusion is the most likely limiting factor for Cai bonic anhydrase, Catalase, and Penicillinase because their KciaIKm is on the order of 10 Chymotrypsin and lysozyme are most likely limited by something other than diffusion. 

Exchange of C02 with red cells and plasma necessarily involves movements of C02 as well as of ions such as Cl, HC03", and OH across the cell membrane to achieve final equilibrium. Carbonic anhydrase is an important factor in the kinetics of these exchanges, and the confinement of this enzyme to the inside of the red cell limits its effectiveness as a catalyst in a cell suspension (14). 

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