Carbon-13 nuclear magnetic spectra, recording

The carbon-13 nuclear magnetic resonance spectrum of a 4.7% (w/v) solution of dorzolamide hydrochloride was obtained in deuterated dimethyl sulfoxide, and is shown in Figure 9. The spectrum was recorded using a Brucker model AM-400 NMR spectrometer. The band assignments were referenced relative to dimethyl sulfoxide-ds (39.5 ppm), and the carbon atom assignments (using the same numbering system as just described) are collected in Table 5. 

Nuclear magnetic resonance spectroscopy Measures the absorption of light energy in the radio-frequency portion of the electromagnetic spectrum. NMR spectroscopy furnishes indirect information about the carbon skeleton of organic molecules. In C NMR peaks corresponding to all carbon atoms are recorded. 

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