Carbon nano tube limitations

Abstract— Molecular techniques have become the most important methods of detecting bacterial and viral pathogens. However, current genomic extraction methods are currently limited in term of automation. In this study, carbon nano-tube was used as the vector to trap DNA and RNA molecules. The capability of carbon nano-tube to trap DNA and RNA was evaluated using samples (TB and HBV for DNA extraction and HCV for RNA extraction) with known quantities and compared to the previously established BOOM method for DNA extraction and Trizol-LS method for RNA extraction. No log difference was observed between the two methods of extraction. Carbon nano-tube is a novel material with potent for automation in molecular diagnosis. 

Evaluation of the novel carbon nano-tube method the capability of the novel carbon nano-tube extraction method was evaluated qualitatively and quantitatively by comparison the results of PCR that were carried out on the extracted genomic samples get from the novel carbon nano-tube based extraction and from the BOOM for DNA or Trizol-LS for RNA extraction. The PCR s limit of detection (LOD) of the target pathogen in the serial dilutions of the bacterial suspension (M. tuberculosis) and the strong positive samples (HBV positive sera) was also applied to evaluate quantitatively the capacity of the novel carbon nano-tube extraction method. 

The LOD (limit of detection). Testing on the serial dilution of the M. tuberculosis suspension and the serial dilution of the HBV-DNA positive sera, the LOD of BOOM method versus carbon nano-tubes method were the same (figure 1). 

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