Carbodiimide Coupling to Phosphatidylethanolamine Lipid Derivatives

Conjugation via Carbodiimide Coupling to Phosphatidylethanolamine Lipid Derivatives 

Prepare liposomes containing PE by any desired method. For instance, the common recipe mentioned in Section 1 mixing PC cholesterol PG PE in a molar 

Add the protein or peptide to be conjugated to the liposome suspension. The protein may be dissolved first in PBS, pH 7.2, and an aliquot added to the reaction lipid mixture. The amount of protein to be added can vary considerably, depending on the abundance of the protein and the desired final density required. From 1 mg protein per milliliter liposome suspension to about 20 mg protein/ml can be reacted. 

Add 10 mg EDC per milliliter of lipid/protein mixture. Solubilize the carbodiimide using a vortex mixer. 

React for 2 h at room temperature. If Uposome aggregation or protein precipitation occurs during the cross-linking process, scale back the amount of EDC added to the reaction. 

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