Capillary zone electrophoresis electro-osmosis

Capillary electrophoretic methods including open-column zone electrophoresis, disc electrophoresis in gels, isotachophoresis and isoelectric focusing have received considerable attention from the analytical community over the last three or four years (80, 81, 82). In capillary zone electrophoresis (CZE), nanogram quantities of sample are placed in a silica capillary, 50 to 300 miaons in diameter and 50 to 100 cm long. Since the small dimensions of the capillary allow for efficient removal of Joule heat, electrical fields up to 350 V/cm can be applied. Under the influence of the field, sample components separate by zone electrophoresis while they are carried downstream by electro-osmosis. 

Figure 7 Capillary zone electrophoresis. The arrows indicate the direction and force of electro-osmotic flow (electroendo-osmosis).

Factors Affecting Ionic Migration. Effect of Temperature. pH and Ionic Strength. Electro-osmosis. Supporting Medium. Detection of Separated Components. Applications of Traditional Zone Electrophoresis. High-performance Capillary Electrophoresis. Capillary Electrochromatography. Applications of Capillary El ectrochromatography. ... 

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