Capillary isotachophoresis equipment used

Capillary isotachophoresis is a rapid, accurate, and potential detection technique for TTX. A small amount of TTX in contaminated extracts can be determined by this method (Shimada et al, 1983). It is performed using a cationic system, as TTX exists as cation under acidic and neutral conditions. Conditions for capillary isotachophoresis composed of 5 mmol/liter potassium acetate (pH 6.0) as an electrolyte, containing 0.2% Triton X-100 and 0.5 volume of dioxane, and 10 mmol p-alanine adjusted to pH 4.5 with acetic acid as a terminating electrolyte. When TTX is applied to isotachophoretic analyzer (Shimadzu IR-2A) equipped with a potential gradient 0.32, it is eventually monitored by the detector. PU is expressed as (PGs-PGl), where PGs, PGl, and PGt stand for potential gradient values for sample, leading ion, and... 

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