Capillary electrophoresis temperature effect

Parameters that should be tested in HPLC method development are flow rate, column temperature, batch and supplier of the column, injection volume, mobile phase composition and buffer pH, and detection wavelength [2], For GC/GLC methods, one should investigate the effects of column temperature, mobile phase flow rate, and column lots or suppliers [38], For capillary electrophoresis, changes in temperature, buffer pH, ionic strength, buffer concentrations, detector wavelength, rinse times, and capillaries lots and supplier should be studied [35, 36], Typical variation such as extraction time, and stability of the analytical solution should be also evaluated [37],... 

Factors Affecting Ionic Migration. Effect of Temperature. pH and Ionic Strength. Electro-osmosis. Supporting Medium. Detection of Separated Components. Applications of Traditional Zone Electrophoresis. High-performance Capillary Electrophoresis. Capillary Electrochromatography. Applications of Capillary El ectrochromatography. ... 

A novel capillary electrophoresis method using solutions of non-crosslinked PDADMAC is reported to be effective in the separation of biomolecules [211]. Soil studies conducted with PDADMAC report the minimization of run-off and erosion of selected types of soils [212]. In similar studies, PDADMAC has found to be a good soil conditioner [213]. The use of PDADMAC for the simultaneous determination of inorganic ions and chelates in the kinetic differentiation-mode capillary electrophoresis is reported by Krokhin [214]. Protein multilayer assemblies have been reported with the alternate adsorption of oppositely charged polyions including PDADMAC. Temperature-sensitive flocculants have been prepared based on n-isopropylacrylamide and DADMAC copolymers [215]. A potentiometric titration method for the determination of anionic polyelectrolytes has been developed with the use of PDADMAC, a marker ion and a plastic membrane. The end-point is detected as a sharp potential change due to the rapid decrease in the concentration of the marker due to its association with PDADMAC [216]. 

Figure 5 Ultrafast SNP analysis by primer extension and capillary electrophoresis. Upper panel electropherogram of the mutant homozygote (G-mutation) with the 19-mer primer peak and the 26-mer product Middle panel electropherogram of the wild type with the primer and the 35-mer product Lower panel electropherogram of the heterozygote with all three peaks (19-, 26-, and 35-mers). Conditions capillary, = 10 cm (effective) (L = 30 cm), i.d. 75 pm separation matrix and running buffer, 10% PVP (MW 1,300,000) in 1 xTBE applied voltage, 20 kV injection, 30 s/10 kV temperature, 30°C. (Reproduced with permission from Ref. 64.)...

Identification of sample components based solely on migration time in capillary electrophoresis (CE) requires reproducibilities not normally obtained. These are caused, mainly, by two effects temperature effects and electro-osmotic affects. Migration times in CE are determined by the electro-osmotic velocity Ueof and effective electrophoretic migration velocity Ueef the net migration velocity Vt is the vector sum of both velocities ... 

Temperature Effects on Mobility, Selectivity, and Resolution in Capillary Electrophoresis... 

Ren, J. and Ueland, P. M. Temperature and pH effects on single-strand conformation polymorphism analysis by capillary electrophoresis. Hum. Mutat., 13, 458, 1999. 

Knox JH, McCormack KA (1994) Temperature effects in capillary electrophoresis. 1. Internal capillary temperature and effect upon performance. Chromatographia 38 207-214... 

Figure 7 CZE separation of hGH and its derivatives. CZE performed in FS capiiiary (i.d. 50 pm, totai iength 105cm, effective iength (to detector) 81.5cm BGE lOmmoii" Tricine, 5.8mmoii morphoiine, 20mmoii NaCi, pH8.0 eiectric fieid intensity 300Vcm current 20pA, temperature 24°C. 1, hGH 2, (desamido-149)- and (desamido-152)-hGH 3, (didesamido-149-152)-hGH. Mesityl oxide, electroosmotic fiow marker. (Reprinted with permission from Grossman PD, Coiburn JC, Lauer HH, et al. (1989) Application of free-solution capillary electrophoresis to the anaiyticai scaie separation of proteins and peptides. Analytical Chemistry 61(11) 1186-1194 American Chemical Society.)...

In capillary electrophoresis, where Joule heat is produced, a change in the effective mobilities of migrating ions can be observed and excessive elevation of the temperature can cause the destruction of thermolabile compounds, e.g., denaturation of proteins. For estimation of the mean temperature of zones T, equation (27) can be used ... 

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