Protein cap-binding

Waskiewicz, A. J., Johnson, J. C., Penn, B., Mahalingam, M., Kimball, S. R., and Cooper, J. A. (1999). Phosphorylation of the cap-binding protein eukaryotic translation factor 4E by protein kinase Mnkl in vivo. Mol. Cell. Biol. 19, 1871—1880. 

Izaurralde, E., Lewis, J., McGuigan, C., Jankowska, M., Darzynkiewicz, E., and Mattaj, I. W. (1994). A nuclear cap binding protein complex involved in pre-mRNA splicing. Cell 78, 657-668. 

Goyer, C., Altmann, M., Trachsel, H., and Sonenberg, N. (1989). Identification and characterization of cap-binding proteins from yeast. J. Biol. Chem. 264, 7603—7610. 

The cap-binding protein eIF4E promotes folding of a functional domain of yeast translation initiation factor eIF4Gl.J. Biol. Chem. 274, 21297—21304. 

Sonenberg, N. (1981). ATP/Mg++-dependent cross-linking of cap binding proteins to the 5 end of eukaryotic mRNA. Nucleic Acids Res. 9, 1643—1656. 

About 1.5-2 hours after infection the first viral structural proteins can be detected in cells efficiently infected by SFV (see Kaariainen and S6-derlund, 1978). By 3-4 hours the synthesis of host cell proteins is shut off. The mechanism of host protein shutoff is not yet known, but studies by van Steeg (1982) suggest that it is the viral capsid protein itself which is responsible for the selective inhibition of host protein synthesis. The capsid protein seems to reduce the activity of the initiation factors elF-4B and CAP binding protein below levels necessary for the formation of the 80 S initiation complex from host mRNA. Translation of the viral 26 S RNA is, however, unaffected. More studies are clearly needed to substantiate this interesting possibility. 

Many antibiotics, which inhibit protein synthesis, do not bind to ribosomes but block any of a variety of vital chemical processes needed for growth. Among them are pseudomonic acid, which inhibits isoleucyl-tRNA synthetase from many gram-positive bacteria.1111/VV Rapamycin, best known as an immunosuppressant (Box 9-F), inhibits phosphoinositide-3-kinase and also phosphorylation of the cap-binding protein 4G, a component of the eukaryotic initiation factor complex (Fig. 29-11 ).ww The bacterial enzyme peptide deformylase, which is absent from the human body, has been suggested as a target for design of synthetic antibiotics. 01... 

The structure of an unrelated cap-binding protein (VP39) has been determined in its apo form and in the presence of various cap analogues... 

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